Anti-Anemic Effect of Breadfruit (Artocarpus altilis) Leaf Ethanolic Extract in Sprague Dawley Rats

Abstract

This study is about investigating the anti-anemic effects of various concentrations (25%, 50%, 75%, and 100%) of ethanolic extract from the leaves of breadfruit (Artocarpus altilis) on the hemoglobin concentration and red blood cell count on Sprague Dawley rats with carboplatin-induced anemia. It was done using randomized trial experimental research design using thirty (30) male Sprague Dawley randomly distributed into six groups (n=5) treated with varying concentrations (25%, 50%, 75%, and 100%), positive and negative controls. Based on the statistical analysis, the findings of this study showed that only Group 3 treated with 25% concentration did not reach the minimum threshold of hemoglobin concentration while all of the concentration yielded normal results in terms of red blood cell count. There was a significant difference in the anti-anemic effect among varying concentrations of the Breadfruit (Artocarpus altilis) leaf ethanolic extract in terms of hemoglobin concentration, that is, both 75% and 100% concentration yielded higher Hemoglobin concentration than 25%. Also, for red blood cell count where 25% concentration significantly differs from all the other concentrations, yielding lower RBC count than all other concentration levels. There was no significant difference in the anti-anemic effects of 75% and 100% concentrations when compared with positive control, in terms of hemoglobin concentration. Whereas negative control yields a significantly lower red blood count than the 50%, 75%, and 100% concentration levels of leaf extract.