Evaluation of the Phytochemical Components and Antimicrobial Property of Gracilaria edulis Extracts Against Selected Aquaculture Pathogenic Bacteria
DOI:
https://doi.org/10.5281/zenodo.20710764Keywords:
aquaculture pathogenic bacteria, ethanolic crude extract, methanolic crude extract, paper disc diffusion method, zone of inhibitionAbstract
With pathogenic bacteria in aquaculture becoming increasingly resistant to antibiotics, there is a compelling need to look into bioactive chemicals present in seaweed as novel treatment options for fish infections. The purpose of this study was to evaluate the phytochemical characteristics of G. edulis extracts and its antimicrobial activities against selected aquaculture pathogenic bacteria. Phytochemical screening of G. edulis was carried out following qualitative test tube screening methods. The antimicrobial activities of the methanolic and ethanolic extracts of G. edulis were tested using the paper disc agar diffusion method against Aeromonas hydrophila, Escherichia coli, and Staphylococcus aureus. Amoxicillin and distilled water were used as a positive and negative control respectively. Based on the phytochemical analysis, the methanolic crude extract of G. edulis contained tannins and flavonoids while the ethanolic crude extract included only tannins. Further, the results of the antimicrobial assay test showed that both methanolic and ethanolic extracts of G. edulis inhibited the bacteria with inhibitory activity comparable to that of the positive control (Amoxicillin). The study therefore suggests that G. edulis can further be investigated for the possible formulation of therapeutics and drugs in light of its potential as an antibacterial agent.
References
Afonso, C., Correia, A. P., Freitas, M. V., Mouga, T., & Baptista, T. (2021). In vitro evaluation of the antibacterial and antioxidant activities of extracts of Gracilaria gracilis with a view into its potential use as an additive in fish feed. Applied Sciences, 11(14), 6642. https://doi.org/10.3390/app11146642
Alsenani, F., Tupally, K., Chua, E., Eltanahy, E., Alsufyani, H., Parekh, H., & Schenk, P. (2020). Evaluation of microalgae and cyanobacteria as potential sources of antimicrobial compounds. Saudi Pharmaceutical Journal, 28(12), 1834–1841. https://doi.org/10.1016/j.jsps.2020.11.010
Andriani, Y., Syamsumir, D. F., Yee, T. C., Harisson, F. S., Herng, G. M., Abdullah, S. A., Orosco, C. A., Ali, A. M., Latip, J., Kikuzaki, H., & Mohamad, H. (2016). Biological activities of isolated compounds from three edible Malaysian red seaweeds, Gracilaria changii, G. manilaensis and Gracilaria sp. Natural Product Communications, 11(8), 1117–1120. https://journals.sagepub.com/doi/abs/10.1177/1934578X1601100822
Arfatahery, N., Mirshafiey, A., Abedimohtasab, T. P., & Zeinolabedinizamani, M. (2015). Study of the prevalence of Staphylococcus aureus in marine and farmed shrimps in Iran aiming the future development of a prophylactic vaccine. Procedia in Vaccinology, 9, 44–49. https://doi.org/10.1016/j.provac.2015.05.008
Assaw, S., Rosli, N. L., Azmi, N. A. M., Mazlan, N. W., & Ismail, N. (2018). Antioxidant and antibacterial activities of polysaccharides and methanolic crude extracts of local edible red seaweed Gracilaria sp. Malaysian Applied Biology, 47(4), 135–144.
Banu, K. S., & Cathrine, L. (2015). General techniques involved in phytochemical analysis. International Journal of Advanced Research in Chemical Science, 2(4), 25–32. https://www.arcjournals.org/pdfs/ijarcs/v2-i4/5.pdf
Bolanos, J., Baleta, F., & Cairel, J. (2017). Antimicrobial properties of Sargassum spp. (Phaeophyceae) against selected aquaculture pathogens. International Journal of Current Microbiology and Applied Sciences, 6(2), 1024–1037. https://www.researchgate.net/publication/313624449_Antimicrobial_Properties_of_Sargassum_spp_Phaeophyceae_against_Selected_Aquaculture_pathogens
Čagalj, M., Skroza, D., Razola-Díaz, M., Verardo, V., Bassi, D., Frleta, R., Mekinić, I., Tabanelli, G., & Šimat, V. (2022). Variations in the composition, antioxidant and antimicrobial activities of Cystoseira compressa during seasonal growth. Marine Drugs, 20(1). https://doi.org/10.3390/md20010064
Dayuti, S. (2018). Antibacterial activity of red algae (Gracilaria verrucosa) extract against Escherichia coli and Salmonella typhimurium. IOP Conference Series: Earth and Environmental Science, 137(1), 012074. https://iopscience.iop.org/article/10.1088/1755-1315/137/1/012074
Escobido, H. S., Orbita, M. L., & Orbita, R. (2016). Evaluation of the biochemical and phytochemical components of green seaweed Enteromorpha intestinalis (Linnaeus) in Initao, Misamis Oriental, Mindanao, Philippines. International Journal of Biosciences, 9(4), 114–122. https://doi.org/10.12692/ijb/9.4.114-122
Firdausy, V., Surdano, & Pramono, H. (n.d.). Activity test of crude extract Gracilaria sp. on Propionibacterium acnes growth inhibition in vitro.
Ghannadi, A., Shabani, L., & Yegdaneh, A. (2016). Cytotoxic, antioxidant and phytochemical analysis of Gracilaria species from Persian Gulf. Advanced Biomedical Research, 5, 139. https://doi.org/10.4103/2277-9175.187373
Gonelimali, F. D., Lin, J., Miao, W., Xuan, J., Charles, F., Chen, M., & Hatab, S. R. (2018). Antimicrobial properties and mechanism of action of some plant extracts against food pathogens and spoilage microorganisms. Frontiers in Microbiology, 9, 1639. https://doi.org/10.3389/fmicb.2018.01639
Guevarra, B., Claustro, A., Madulid, R., Aguinaldo, A., Espeso, E., Nonato, M., Quinto, E., Santos, M. A., De Castro-Bernas, G., Gonzales, R., Del Castillo Solevilla, R., & Ysrael, M. (2005). A guidebook to plant screening: Phytochemical and biological (pp. 23–98).
Kantida, R. S., Asha, K. R. T., & Sujatha, S. (2012). Influence of bioactive compounds from seaweeds and its biocidal and corrosion inhibitory effect on mild steel. Research Journal of Environmental Toxicology, 6, 101–109. https://doi.org/10.3923/rjet.2012.101.109
Levy, S. B., & Marshall, B. (2004). Antibacterial resistance worldwide: Causes, challenges and responses. Nature Medicine, 10(12), 122–129. https://doi.org/10.1038/nm1145
Miao, L., & Qian, P. Y. (2005). Antagonistic antimicrobial activity of marine fungi and bacteria isolated from marine biofilm and seawaters of Hong Kong. Aquatic Microbial Ecology, 38, 231–238. https://doi.org/10.3354/ame038231
Pérez, M. J., Falqué, E., & Domínguez, H. (2016). Antimicrobial action of compounds from marine seaweed. Marine Drugs, 14(3), 52. https://doi.org/10.3390/md14030052
Qari, R., & Khan, A. R. (2019). Studies on antibacterial and phytochemical analysis of Gracilaria corticata (J. Agardh), Gracilaria dentata (J. Agardh), and Gracilaria pygmaea (Borgesen) against diarrheal causing pathogen E. coli and Salmonella typhi. Acta Scientific Microbiology, 2(3), 3–10. https://www.researchgate.net/publication/331431002
Rashid, M. M., Hossain, M. S., & Ali, M. F. (2013). Isolation and identification of Aeromonas hydrophila from silver carp and its culture environment from Mymensingh region. Journal of the Bangladesh Agricultural University, 11(2), 373–376. https://doi.org/10.3329/jbau.v11i2.19943
Rayapu, L., Makkar, F., Chakraborty, K., & Valluru, L. (2017). Phytochemical evaluation and antimicrobial activity of Gracilaria opuntia: An important anti-diabetic red marine macroalgae. International Journal of Current Pharmaceutical Research, 9(6), 37–41. https://www.researchgate.net/publication/321060980_PHYTOCHEMICAL_EVALUATION_AND_ANTIMICROBIAL_ACTIVITY_OF_GRACILARIA_OPUNTIA_AN_IMPORTANT_ANTIDIABETIC_RED_MARINE_MACROALGAE
Reza, R., Shipa, S. A., Naser, N., & Miah, F. (2021). Surveillance of Escherichia coli in a fish farm of Sylhet, Bangladesh. Bangladesh Journal of Zoology, 48(2), 335–346. https://doi.org/10.3329/bjz.v48i2.52373
Salem, W. M., Galal, H., & Nasr Eldeen, F. (2011). Screening for antibacterial activities in some marine algae from the Red Sea (Hurghada, Egypt). African Journal of Microbiology Research, 5(15), 2160–2167. http://www.academicjournals.org/ajmr
Sobuj, M. K. A., Islam, M. A., Islam, M. S., Islam, M., Mahmud, Y., & Rafiquzzaman, S. M. (2021). Effect of solvents on bioactive compounds and antioxidant activity of Padina tetrastromatica and Gracilaria tenuistipitata seaweeds collected from Bangladesh. Scientific Reports, 11, 19082. https://doi.org/10.1038/s41598-021-98461-3
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Journal of Pure and Applied Sciences

This work is licensed under a Creative Commons Attribution 4.0 International License.
All articles published by the Journal of Pure and Applied Sciences are licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).

